Protein analysis, protein concentration, protein sizing, antibody analysis, antibody qc, protein quality control, ProteoAnalyzer, enzyme, cell lysate analysis, protein expression, antibodies, protein qc

New Agilent ProteoAnalyzer System: Automate Your Protein Analysis

The Agilent ProteoAnalyzer system is an automated quality control (QC) instrument designed to provide accurate and precise quality measurements of protein samples. The system analyzes proteins based on migration time and fluorescence intensity using parallel capillary electrophoresis with sodium dodecyl sulfate (CE-SDS). Rapid and consistent sizing and quantification results can be achieved for both reduced and nonreduced protein samples.

The ProteoAnalyzer system analyzes up to 12 samples simultaneously in one run in approximately 30 minutes. Accurate sizing can be achieved for both small and large samples, ranging in size from 10 to 240 kDa. Samples can be detected over a wide concentration range, from 2 to 2,000 ng/µL. The discrete individual capillaries are rejuvenated between every separation allowing for the analysis of diverse samples including monoclonal antibodies, biosimilars, protein purification fractions, and crude cell lysates.

The ProteoAnalyzer is a robust system, well-suited for protein applications in biotherapeutics and synthetic biology workflows, enabling scientists to efficiently perform various protein quality and quantity assessments using the Agilent Protein Broad Range P240 kit.

Key advantages:

• Analyze challenging sample types—using discrete capillaries for separations, purified antibodies, secreted proteins, membrane proteins, and crude lysates can be consistently and simultaneously assessed

• Increase lab efficiency through automation—automated instrument preparation, separation, and streamlined protein labeling procedures allow you to analyze more samples per day

• Get consistent results between runs—the ProteoAnalyzer system automatically cleans, reconditions, and refills capillaries between every sample, leading to reliable results

• Take advantage of digital data—automated data processing and exportable data files reduce analysis time and simplify data archiving and recovery

The ProteoAnalyzer system adds efficiency, versatility, and reliability, particularly in your protein QC workflows.

For Research Use only. Not for use in diagnostic procedures. PR7001-2107

Product Specifications

Sizing range: 10 to 240 kDa and 10 to 200 kDa, depending on method used

Typical Sizing Accuracy: < 15% for BSA, CAII and < 10% for BSA, CAII, depending on method used

Typical resolution: <10% molecular weight resolution between 15 to 150 kDa (based on ladder)

Sizing Precision: <5% - <10% for standard proteins, depending on method used

Quantitative Range: 2 ng/µL to 2,000 ng/µL for BSA in PBS

Sensitivity (S/N >3): 1 ng/µLfor BSA, CAII in PBS

Quantification Reproducibility: <15 %CV for 20-2,000 ng/µL and <25 %CV for 2-20 ng/µL BSA

Total Run Time: 30 minutes

Samples Per Run: 11 samples + ladder in well 12