New 1-Step Rt-qPCR Master Mix From Thermo Fisher Scientific Helps Increase Productivity and Performance

Labs incorporating automation into their qPCR workflows need assurance that their reagents are stable during reaction setup and testing. However, existing 1-Step RT-qPCR master mixes experience lower sensitivity, specificity, and consistency when assembled reactions are left at room temperature for extended periods. Unlike other 1-Step RT-qPCR Master Mixes available on the market, the new Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix from Thermo Fisher Scientific maintains performance at room temperature for a full work day (>8hours), providing more flexibility for labs to incorporate 1-Step RT-qPCR into their automated workflow.

The TaqPath DuraPlex 1-Step RT-qPCR Master Mix is now available for biopharma and clinical diagnostics labs that need a rapid, sensitive, and reproducible method for developing and testing high-throughput molecular diagnostic or bioprocess quality control assays. This benchtop-stable, single-tube master mix is optimized for detection of virus and bacterial pathogens even in the presence of challenging PCR inhibitors. Labs can now confidently automate their 1-Step RT-qPCR workflows, without loss of sensitivity, specificity, or reproducibility, with the ability to multiplex up to 6 targets in a single reaction.

“Overall, we were quite pleased with the performance of TaqPath DuraPlex 1-Step RT-qPCR Master Mix and found that it performed considerably better than a similar on-market formulation we tested in conjunction. Specifically, we found the product to perform significantly better in both inhibitor tolerance and in-channel multiplexing, especially at low copy number,” said Alison Todd, AM, FTSE, PhD, Chief Scientific Officer & Founder, SpeeDx Pty Ltd.