Look Deeper Into the Metabolome With High-Resolution GC-MS

Look deeper into the metabolome with high-resolution GC-MS Gas chromatography-mass spectrometry (GC-MS) is a core analytical technique used to identify and characterize small molecule metabolites. While GC-MS remains a gold-standard approach, achieving sufficient resolution while maintaining analytical sensitivity remains a challenge in biopharmaceutical workflows. This infographic explores the role of high-resolution GC-MS in biopharmaceutical and metabolomics research. It also highlights the latest, sensitive solutions for a greater depth of analysis. Pharmaceutical applications include: Metabolism Toxicology studies Biomarker Drug discovery discovery The GC-MS analysis Data collection workflow While GC-MS workflows vary depending on their applications and objectives, most share common elements including data collection, annotation and chemical structure elucidation. Peak detection Statistical analysis Molecular confirmation Structure elucidation Resolving a common challenge Mass resolution refers to a system’s ability to distinguish between adjacent ion peaks. Ions with similar mass-to-charge ratios (m/z) may produce peaks that are difficult to distinguish if an instrument is not capable of a high mass resolution (i.e., it has a low resolving power or RP).1 .1 Low resolution (15,000 RP) Compound A Higher resolution Matrix (60,000 RP) Compound A Highest resolution Matrix (240,000 RP) An RP of >60,000 can help you: Avoid false negatives Increase discovery power Analyse more complex samples Achieve more accurate quantification Greater resolution for confident compound identification The Thermo Scientific™ Orbitrap Exploris™ GC 240 mass spectrometer can help achieve an unprecedented depth of analysis through c™ s™ the highest data quality. Capable of a maximum resolving power of 240,000 (FWHM at m/z 200) this benchtop device offers increased accuracy, sensitivity, selectivity and flexibility as you look deeper into the metabolome. High resolving Greater capability sensitivity Analytical Targeted and power and untargeted flexibility workflows While increased resolving power can result in decreased instrument sensitivity in some systems, the Orbitrap Exploris™ GC s™ 240 offers users the highest sensitivity at any resolution. This makes it the perfect solution for low-level targeted screening, quantification and profiling in complex samples. Other systems Orbitrap Exploris GC 240 Resolving power Resolving power Sensitivity Sensitivity With the Orbitrap Exploris™ GC 240, an increased RP results in improved mass accuracy. This means that small mass differences s™ can be accurately detected, giving you confidence in your data. Resolving power Resolving power . . Mass error Mass error . . • Increase accuracy (<1 ppm) • Reduce matrix interference • Increase confidence in compound identification • Reduce false positives Compound A Compound B Compound C 15,000 60,000 240,000 Resolving power References 1. Cooper J, Roberts D, Cojocariu C. Mass resolving power of 240,000: for confident compound identification. ThermoFisher Scientific. 2020 https://assets.thermofisher.com/TFS-Assets/CMD/Technical-Notes/tn-10730-gc-ms power- confident-compound-identification-tn10730-en.pdf Accessed June 2023. Discover a greater depth of analysis with high-resolution GC-MS For Research Use Only. Not for use in diagnostic procedures. © 2023 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. in-002417-na-en Mass accuracy (ppm)