Characterization of Sequence Modifications in Monoclonal Antibodies
Monoclonal antibodies are considered the most rapidly growing class of therapeutics. Critical quality attributes such as sequence assessment, charge and size variants must be thoroughly monitored since they can alter the biological activities of the product.
Hyphenation of size-exclusion chromatography and cation-exchange chromatography with native mass spectrometry have been widely used to provide a more complete characterization of mAb populations.
Top-down and middle-down mass spectrometry are also emerging strategies for deciphering the structure of proteoforms and the presence of post-translational modifications.
In this webinar, our expert speaker will discuss how these complementary techniques can be performed on the ZenoTOF instrument to localize modifications on therapeutic mAbs.
Attend this webinar to:
- Discover how to implement non-denaturing workflows to characterize biotherapeutics
- Learn to monitor size and charge variants with very limited sample preparation
- Hear how a method for mAb characterization was developed
- Explore why localizing post-translational modifications and identifying charge variants is crucial
