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Using a novel quantitative real-time PCR assay, specific to the mature product, the expression level of a selected group of miRNAs was measured across a set of 30 primary adult acute myeloid leukemia (AML) with a normal karyotype.

In the proteomics area with the increasing capacity of the mass spectrometers, sample preparation is becoming a limiting step for protein identification. Magnetic particle based technology provides a rapid and easy solution for automation of the sample preparation step.

In the proteomics area with the increasing capacity of the mass spectrometers, sample preparation is becoming a limiting step for protein identification. Magnetic particle based technology provides a rapid and easy solution for automation of the sample preparation step.

MolPAGE (Molecular Phenotyping to Accelerate Genomic Epidemiology) is an EU consortium with almost twenty collaborating universities and companies throughout Europe. One of the aims of MolPAGE is to find early onset biomarkersfor type 2 diabetes (T2DM) and cardio-vascular diseases(CVD).

DeCyder™ MS is a novel software program for fully automated differential expression analysis based on LC-MS/MS data including detection, quantitation, sample to sample comparison and statistical data evaluation. Results can be interactively confirmed against original raw data through 2D and 3D data visualizations.

By representing LC-MS data as a 2D-image as demonstrated in the novel DeCyder™ MS software, it is possible to identify phenomena, which are very difficult to recognize using conventional LC-MS data representation.

In this work, a biocompatible nano liquid chromatography (LC) system, Ettan™ MDLC, was used for separating tryptic peptides from brain tissue by cation exchange (SCX) to enrich the phosphopeptides followed by reversed-phase chromatography (RPC). The phosphopeptides were detected by neutral loss MS.

This work demonstrates the development and validation of a novel clinical proteomics approach in which specific protein targets can be expeditiously and selectively isolated from acomplex biological fluid for MS characterization.

By using the detection, matching, and visualization approach ofDeCyder™ MS were retention time, precursor mass, and the topology of the intensity profile is utilized in combination with the matching of tandem mass spectra, it is possible to achieve repeat analysis with a very high reproducibility.